Agricultural and rural informatization is the leading force and important engine to drive agricultural and rural modernization, promote agricultural efficiency and increase farmers ' income. Based on the panel data of 31 provinces across the country from 2000 to 2019, and taking the construction pilot project of the national rural and agricultural informatization demonstration province as a quasi-natural experiment, the multi-period difference-in-differences model is used to evaluate the income increase effect of agricultural and rural informatization. The results show that the implementation of agricultural and rural informatization policy can significantly improve the per capita disposable income of rural residents. The effect of agricultural and rural informatization on farmers' income has regional heterogeneity, which is embodied in the promotion effect of agricultural and rural informatization policy in the western region on farmers' income significantly stronger than that in the central and eastern regions. Therefore, we should further improve the policy system, clear the policy direction, do a good job in policy implementation and pay attention to the policy tilt, so as to enhance the effect of the agricultural and rural informatization policy on increasing farmers' income.